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. 2018 Apr 24;9:531. doi: 10.3389/fpls.2018.00531

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Copyright © 2018 Dechorgnat, Francis, Dhugga, Rafalski, Tyerman and Kaiser.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic comparison of F1 BK3 (gray) and parental B73 (white) and F44 (black) roots. (A) Crown root measurements normalized to B73. (B) Low affinity transport (LATS+HATS) of 2.5 mM of nitrate (NO₃^-) and ammonium (NH₄⁺). (C) NO₃^- and NH₄⁺ LATS+HATS capacities in the PR, SR, and CR root tissues normalized to B73. (D) Measurements of nitrate (NO₃^-), nitrite (NO₂^-), and ammonium content (NH₄⁺) normalized to B73. (E) Analysis of nitrate reductase activity (NRA), nitrite reductase activity (NiR), and asparagine synthetase activity (ASN) normalized to B73. (F) ZmNPF6.6 and ZmAMT1.1A gene expression in the PR, SR, and CR root tissues normalized to B73. Values are means (±SE) from 3 to 8 individual plants. Asterisks indicate significant differences with B73 at ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.005 (Student’s t-test). Different letters indicate significant differences among means at P < 0.05 (ANOVA).