Table 1.

Excitation and emission wavelengths of commonly used fluorescent dyes

Visualization molecules	Abbreviation	2 Photon excitation	Conventional excitation	Emission
Fluorescent proteins, jellyfish-derived	eBFP	780	380	440
	eCFP	860–920	433	475
	eGFP	880–930	488	509
	eYFP	960	513	527
Fluorescent proteins; coral-derived	Kaede (before conversion)	730	508	518
	Kaede (after conversion)	730	572	582
	tdTomato	900–1000	554	581
	DsRed	930–990	558	583
	mCherry	760 or 900–1000	587	610
Chemical dyes	CMF2HC	780–800	371	464
	CFSE	780–820	492	517
	CMFDA	800	492	517
	FITC	780–800	494	520
	CMTMR	780–820	541	565
	SNARF	700–810	563	639
	CMTPX	780–800	577	602
	Texas Red	780–920	595	615
Calcium indicator	Indo-1	700	346	475
	Fura-2	700–850	363	512
	Fura-4F	700–850	366	511
	Fluo-5N	800	520	475
Organelle visualization	Hoechst	780	350	461
	DAPI	700	358	461
	FM 1–43	830	480	565
	Rhodamine 123	780–860	507	529
	Dil	700	549	565
	Sulforhodamine 101	840–890	586	605
	DiD	780	644	665