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. 2018 May 1;20:8. doi: 10.1186/s12575-018-0075-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Structure of the plasmid p#5. p#5 contains the modified Hfq gene from S. meliloti with sequence (red) for an affinity His-tag to produce Hfq^His. The gene is cloned under the regulation of the native Hfq gene promoter (P_hfqsm) [37]. p#5, a derivative of pBBR1MCS-5 [38], also carries a gentamicin resistance gene (Gm^r), the broad host range replication (rep) origin and the sequence (mob) allowing for conjugal mobilization