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. 2018 May 1;20:8. doi: 10.1186/s12575-018-0075-8

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Hfq complexes smelA075 in M. truncatula nodules. a RT-PCR detected smelA075 from Hfq complexes immunoprecipitated from lysates of M. truncatula nodules 10-dpi (lane 2), 18-dpi (lane 3) and 49-dpi (lanes 4), but failed to detect smelA075 from control samples (lane 5). Markers (lane 1). This experiment was repeated twice and similar results were obtained (data not shown). b smelA075 immunoprecipitated with the Hfq^His was analyzed by qPCR. Relative enrichments were calculated with the 2^-∆∆Ct method [39]. The data are presented as mean fold change ± SEM and normalized to S. meliloti 16S rRNA gene