Fig. 3.

The anti-ITGA2 antibody induced a RhoA-p38 MAPK-mediated apoptotic pathway in AGS cells. a mRNA expression of Ras, RhoA, Bax, p38, Bim, Bcl-2, Apaf-1 and Caspase-9 in AGS cells treated with 0.3 μg of anti-ITGA2 antibodies or isotype control antibodies (negative control) for 48 h were analyzed by RT-PCR. Each analysis was derived from the same experiment and gels were processed in parallel. Cropped gels are displayed to compare gene expressions in different treatment groups. b Densitometric measurements on the intensities of each RT-PCR product was normalized to the mRNA level of GAPDH, and displayed as mean ± standard deviation (S.D). Statistical comparisons were made by two-way ANOVA with Bonferroni comparisons. ** p < 0.01, **** p < 0.0001. Data are representative of three independent experiments. c Summary of the anti-ITGA2 antibody-mediated apoptosis signaling pathway in AGS gastric cancer cells