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. 2018 Mar 9;17(5):6301–6310. doi: 10.3892/mmr.2018.8713

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Copyright: © Qiu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Downregulation of miR-214-5p affected ALP, Runx2, OC and COL1A1 mRNA expression. (A) RT-qPCR was initially performed to confirm successful downregulation of miR-214-5p following transfection. RT-qPCR was subsequently performed to determine the effect of anti-miR-214-5p on (B) ALP, (C) Runx2, (D) OC and (E) COL1 mRNA expression in human bone marrow stem cells. ##P<0.01 vs. negative control group. miR, microRNA; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; OC, osteocalcin; COL1A1, collagen type IV α1 chain; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; negative, negative control transfection group; anti-miR-214-5p group, miR-214-5p downregulation group.