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. 2018 Mar 9;17(5):6301–6310. doi: 10.3892/mmr.2018.8713

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Copyright: © Qiu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — TGF-β inhibitor reversed the effects of miR-214-5p downregulation on the protein expression of COL4A1, TGF-β and p-Smad2. (A) Western blot analysis demonstrated that TGF-β inhibitor reversed anti-miR-214-5p-induced increases in the protein expression of COL4A1, TGF-β and p-Smad2. Densitometric analysis of western blotting results was performed to quantify the protein expression of (B) COL4A1, (C) TGF-β and (D) p-Smad2 following downregulation of miR-214-5p with or without TGF-β inhibitor treatment in human bone marrow stem cells. (E) Following miR-214-5p downregulation with or without TGF-β inhibitor treatment, adipogenic differentiation was assessed using oil red O staining. Magnification, ×100. ##P<0.01 vs. negative control group; **P<0.01 vs. anti-miR-214-5p group. TGF-β, transforming growth factor-β; miR, microRNA; COL4A1, collagen type IV α1 chain; p-Smad2, phosphorylated-Smad2; negative, negative control transfection group; anti-miR-214-5p group, miR-214-5p downregulation group; TGF-β inhibitor group, miR-214-5p downregulation+TGF-β inhibitor group.