Table I.
Gene | Forward | Reverse |
---|---|---|
miR-214-5p | 5′-GGCCTGGCTGGACAGA-3′ | 5′-GTCACATGACAACCCAGCCT-3′ |
ALP | 5′-TGGAGGTTCAGAAGCTCAACACCA-3′ | 5′-ATCTCGTTCTCTGAGTACCAGTC-3′ |
Runx2 | 5′-CCGCACAACCGCACCAT-3′ | 5′-CGCTCCGGCCCACAAATCTC-3′ |
OC | 5′-CAGCGGTGCAGAGTCCAGCAAA-3′ | 5′-GATGTGGTCAGCCAACTCGTCA-3′ |
COL1A1 | 5′-CCTGGAAAGAATGGAGATGATG-3′ | 5′-ATCCAAACCACTGAAACCTCTG-3′ |
PPARγ | 5′-TTATGGAGCCTAAGTTTGAGTTTGC-3′ | 5′-TTGTCTTGGATGTCCTCGATGG-3′ |
CEBPα | 5′-GAAGTCGGTGGATAAGAACAGCA-3′ | 5′-CTCCAACACCTTCTGCTGCGT-3′ |
Adiponectin | 5′-GTCCCTCCACCCAAGGAAACT-3′ | 5′-CTCCTGTCATTCCAGCATCTCC-3′ |
U6 | 5′-CTCGCTTCGGCAGCACATATACT-3′ | 5′-ACGCTTCACGAATTTGCGTGTC-3′ |
GAPDH | 5′-GGGCTGCTTTTAACTCTGGT-3′ | 5′-GCAGGTTTTTCTAGACGG-3′ |
miR, microRNA; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; OC, osteocalcin; COL1A1, collagen type I; PPARγ, peroxisome proliferator-activated receptor γ; CEBPα, CCAAT/enhancer-binding protein α.