Figure 2.

Overexpression of miR-378 promoted cell migration and invasion of cervical cancer. (A) Relative expression of miR-378 was determined by reverse transcription-quantitative polymerase chain reaction. (B) Overexpression in C-33A cells promoted cell migration and knockdown of miR-378 in HeLa cells inhibited cell migration (magnification, ×100). (C) Overexpression in C-33A cells promoted cell invasion and knockdown of miR-378 in HeLa cells inhibited cell invasion (magnification, ×100). (D) Effects of miR-378 on the protein expression of ATG12 were determined by western blot analysis in C-33A cells transfected with GIPZ-miR-378 and HeLa cells transfected with miR-378 inhibitor. (E) Quantitative analysis of the western blot results in Fig. 2D. Each bar represents the grey area of each band and the statistical significance was analyzed from three independent replicates. The data are presented as the mean ± standard deviation. *P<0.05. ATG12, autophagy-related protein 12; CCND1, cyclin D1; miR, microRNA; NC, negative control; pRb, retinoblastoma.