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. 2018 Mar 8;17(5):6465–6471. doi: 10.3892/mmr.2018.8705

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Copyright: © Jung et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Immunohistochemistry for VGLUT1 in the hippocampus of the (A) adolescent PM1, adult (B) PM6 and (C) PM12, and aged (D) PM18 and (E) PM24 groups. VGLUT1 immunoreactivity was observed in the mossy fibers (indicated by MF) of the dentate gyrus as well as the Schaffer collaterals (indicated by SC) and stratum lacunosum-moleculare (indicated by SLM). VGLUT1 immunoreactivity was detected in the inner molecular layer (indicated by iML) in the PM6 and PM24 groups. Scale bar=500 µm. (F) ROD are expressed as a percentage of the value of the VGLUT1 immunoreactivity in the PM1 group in the dentate gyrus, hippocampal CA1 and CA2/3 regions per section of the PM1, PM6, PM12, PM18 and PM24 groups (n=5/group). Data are presented as the mean ± standard error mean. *P<0.05 vs. PM1. GLUT, vesicular glutamate transporter; PM, postnatal month; ROD, relative optical densities.