Figure 6.

Effects of MIF antagonist ISO-1 on the P38MAPK and NF-κB signaling pathway in the lung injury induced by APIP. Lung samples were obtained at 6 h after modeling. MIF, total and phosphorylated P38 and TNF-α in the cytoplasm, as well as NF-κB in the cytoplasm and nucleus were measured by western blot assay. β-actin was used as internal control of cytoplasm, and LaminB was used as nucleus internal control. Densitometry quantification of (A) MIF, (B) p-P38, (C) NF-κB, and (D) TNF-α was evaluated by the Quantity One software. Data are expressed as mean ± SD (n=6). P<0.05 indicates a significant difference between the marked groups. SO, sham operation group; APIP, acute pancreatitis in pregnancy group; ISO-1, ISO-1+APIP group. MIF, macrophage migration inhibitory factor; ISO-1, (S,R)3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester; TNF-α, tumor necrosis factor-α; NF-κB, nuclear factor-κB