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. 2018 Mar 20;17(5):7055–7064. doi: 10.3892/mmr.2018.8792

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Copyright: © Sun et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Western blot analysis of pacemaker cell-associated protein expression in BASCs and WASCs following TBX18 transduction. (A) Representative western blot bands for TBX18, TBX3, HCN4 and Sr in WASCs and BASCs transduced with either AD-TBX18 or AD-GFP. Densitometric analysis was performed to quantify the protein expression of (B) TBX18, (C) TBX3, (D) HCN4 and (E) Sr. Expression levels were normalized to β-actin. Data are presented as the mean ± standard error of the mean n=3. *P<0.05 vs. AD-GFP-BASCs; ^#P<0.05 vs. AD-GFP-WASCs; ^$P<0.05 vs. AD-TBX18-WASCs. BASCs, brown adipose-derived stem cells; WASCs, white adipose-derived stem cells; TBX, T-box; HCN4, hyperpolarization-activated cyclic nucleotide-gated channel 4; Sr, sarcomeric α-actinin; AD, adenovirus; GFP, green fluorescent protein.