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Primer sequences used for reverse transcription-quantitative polymerase chain reaction.
| Gene | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
| β-actin | AGCCATGTACGTAGCCATCC | GCTGTGGTGGTGAAGCTGTA |
| TBX18 | TGCTGTCCCTGCTACACATC | GCTGTAGGTCTCTGCCAAGG |
| HCN4 | TCATCTCCTCCATCCCTGTC | CTGGCCAGGTCATAGGTCAT |
| TBX3 | GCTGCTGCGAACTCTCTTCT | GAAGGTGTCGGAAACTGGAA |
| Sr | TCATCTCAGGTGAACGCTTG | AGATGTCCTGGATGGCAAAG |
TBX, T-box; HCN4, hyperpolarization-activated cyclic nucleotide-gated channel 4; Sr, sarcomeric α-actinin.
