Table I.
Primer sequences.
| Gene | Sequence (5′-3′) | Product length (bp) | Accession number |
|---|---|---|---|
| Leptin-F | CAATGACATTTCACACACGCAG | 204 | NM_013076 |
| Leptin-R | AGATGGAGGAGGTCTCGCAG | ||
| Leptin receptor-F | GTGTCCTTCCTGACTCCGTAG | 119 | NM_012596 |
| Leptin receptor-R | GTTATTCTCTGGAAAGACTGGCT | ||
| β-actin-F | GGCTGTATTCCCCTCCATCG | 154 | NM_007393 |
| β-actin-R | CCAGTTGGTAACAATGCCATGT | ||
| KiSS-1-F | TGGCACCTGTGGTGAACCCTGAAC | 202 | NM_181692 |
| KiSS-1-R | ATCAGGCGACTGCGGCTGGCACAC | ||
| GPR54-F | TTGGGTCCGAACGTGAGCT | 371 | NM_001301151 |
| GPR54-R | CATGTGGCTTGCACCGAGA | ||
| GnRH-F | CCGCTGTTGTTCTGTTGACT | 201 | NM_012767 |
| GnRH-R | GCAGATCCCTAAGAGGTGAA | ||
| LH-F | CCCCATAGTCTCCTTTCCTG | 190 | NM_012858 |
| LH-R | GGATGGTTAGAACACCTGCT | ||
| FSH-F | CATCCTACTCTGGTGCTTGA | 325 | NM_008045 |
| FSH-R | TCTTACAGTGCAGTCGGTGC |
F, forward; R, reverse; KiSS-1, kisspeptin; GPR54, G-protein coupled receptor 54; GnRH, gonadotropin releasing hormone; LH, luteinizing hormone; FSH, follicle-stimulating hormone; bp, base pairs.