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. 2018 Mar 20;17(5):7097–7104. doi: 10.3892/mmr.2018.8784

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Knockdown of miR-133b-5p blocked HPC-induced cardioprotection. (A) The viability of cardiomyocytes was measured by Cell Counting Kit-8 method. (B) Cell injury was determined by lactate dehydrogenase release in the culture medium. (C) Cell apoptosis was measured by Annexin V-FITC/PI flow cytometry and (D) the apoptotic rate is expressed as the percentage of cells at early apoptotic stage (the lower right quadrant). (E) The level of miR-133b-5p was detected by quantitative RT-PCR. Data are presented as mean ± SEM (n=3). ^&P<0.05 vs. HPC. HPC, hypoxic preconditioning; miR, microRNA; ant-miR-133b, miR-133b-5p antagomir; ant-miR NC, antagomir negative control; FITC, fluorescein isothiocyanate; PI, prodium iodide.