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. 2018 Mar 14;17(5):7170–7176. doi: 10.3892/mmr.2018.8733

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — PEDF-induced autophagy contributes to lipid degradation. H9c2 cells were maintained in normoxic or hypoxic conditions for 4 h with or without PEDF (10 nM). RNA interference assays were used to silence PEDF-R expression and autophagy inhibitor 3-MA (5 mM) was added. (A) Representative images of transmission electron microscopy analysis of H9c2 cells LDs. Typical LDs (arrows) were observed. Scale bar, 1 µm. (B) TG levels were quantified with a TG assay kit. *P<0.05. Ctrl, control; LDs, lipid droplets; PEDF-R, pigment epithelial-derived factor receptor; 3-MA, 3-methyladenine; siPEDF, short interfering PEDF RNA; TG, triglyceride.