Figure 3.

5′-Deletion analysis of the pra2 upstream region. The 5′-deletion constructs containing the pra2 upstream region were fused to the promoter-less pBI221-LUC+ plasmid. The numbers refer to the 5′ end of the pra2 upstream fragments from the transcriptional start site in Figure 1. The names of the resulting plasmids are indicated. Equivalent amounts of pBI221-LUC+ plasmid DNA fused to different pra2 upstream regions were introduced into the growing zone of etiolated pea stem by particle bombardment, with the 35S-GUS construct as the internal standard. After bombardment, samples were kept in darkness (D) or under continuous white light (L) for 12 h. Relative activity is defined in Figure 2b. Values are the means of at least four independently bombarded samples with error bars representing se (n ≥ 4). UTR, Untranslated region; NOS, nopaline synthase terminator.