Figure 5.

Red/far-red reversibility of the change in the reporter-enzyme activity. Different kinds of constructs containing the pra2 upstream region were prepared as described in Methods. The names of the resulting plasmids are indicated on the right. White bold line, 93-bp region; thin line, deleted region. Equivalent amounts of each plasmid were introduced into 5- or 6-d-old etiolated seedlings as described in Methods. After bombardment, the plants were exposed to red light for 2 min (R) or far-red light for 5 min immediately after red light for 2 min (R/FR) and then were returned to darkness for 12 h. Dark and far-red controls are indicated as D and F, respectively. Values are the means of 5 to 11 independently bombarded samples, with error bars representing se. Relative activity is defined in Figure 2b. A fine-scale figure of PL4C is shown in the inset. NOS, Nopaline synthase terminator.