Figure 6.

LS analysis to define the 12-bp phytochrome down-regulated element. LS constructs in the pra2 5′-upstream region are shown. The positions of mutated nucleotides are indicated in lowercase and underlined. The WT sequence corresponds to the PL4A construct in Figure 5. The 12-bp phytochrome-responsive element is boxed. Equivalent amounts of each plasmid were introduced into 5- or 6-d-old etiolated seedlings as described. After bombardment, the plants were exposed to red light for 2 min (R) and then returned to darkness for 12 h. D, Dark control. Values are the means of at least five independently bombarded samples, with error bars representing se. Relative activity is defined in Figure 2b.