Figure 7.

Gel-mobility shift assays with the synthetic oligonucleotides. a, Sequences of synthetic oligonucleotides used in this experiment are shown. The 12-bp sequence is boxed. Nucleotides in the MT oligonucleotide that are different from the WT oligonucleotide are indicated in lowercase with arrows. b, Binding of nuclear proteins from pea stems to the WT oligonucleotide and competition with the WT and MT oligonucleotides. The 12-bp-specific bound complex is indicated by the arrow. D and L indicate the nuclear extracts of dark-grown plants and plants exposed to light for 6 h, respectively.