Table 1.

The specific primers used in the present study

Gene regions	Primers/approximate amplicon size	PCR conditions
18S rRNA	For Trichuridae nematodes	1. Pre-incubation 94°C 5 min
	18S965F: 5′-GGCGATCAGATACCGCCCTAGTT-3′	2. Amplification for 35 cycles
	18S1573R: 5′-TACAAAGGGCAGGGACGTAGT-3′ (Guardone et al., 2013)	Denaturation 95°C 30 sec
	PCR product size was 727 bp	Annealing 59°C 30 sec
		Extension 72°C 30 sec
		3. Final extension 72°C 10 min
ITS2	For T. trichiura	1. Pre-incubation 94°C 5 min
	ITS2_tt_F2: 5′-GCTCGTAGGTCGTTGAAG-3′	2. Amplification for 35 cycles
	ITS2_tt_R2: 5′-TAGCCAAGTCGGGTAGT-3′	Denaturation 95°C 30 sec
	For T. suis	Annealing 50°C/54°C* 30 sec
	ITS2_tt_F2: 5′-GCTCGTAGGTCGTTGAAG-3′	Extension 72°C 30 sec
	ITS2_tt_R2_new1: 5′-GGGCAGCTTCCGTACT-3′ (newly designed primers)	3. Final extension 72°C 10 min
	PCR product size of T. trichiura was 325 bp and T. suis was 355 bp

ITS2 = internal transcribed spacers 2; PCR = polymerase chain reaction.

^*Annealing temperature used was 50°C for T. trichiura and 54°C for T. suis.