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. 2018 Mar 13;39(5):2071–2080. doi: 10.3892/or.2018.6310

Figure 1.

Figure 1.

Figure 1.

Expression of CD40L-M induces cellular senescence. (A) CD40-positive NSCLC A549, A549/TR, A549/DDP and H460 cell lines were transfected as indicated and CD40L protein was measured using western blot analysis and (B) quantified with densitometry, respectively. β-tubulin was used as a loading control. (C) Representative imageas of SA-β-gal staining and (D) the percentage of SA-β-gal-positive cells. Data are expressed as the means ± SD of three independent experiments. *P<0.05, **P<0.01 compared to the controls or negative group.