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. 2018 Mar 13;39(5):2071–2080. doi: 10.3892/or.2018.6310

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — ATM/Chk2 is necessary for CD40L-M-induced senescence. (A) p-Chk1 and p-Chk2 expression was determined in CD40L-M-transfected cells by western blot analysis. (B) Quantitative analysis of protein levels. (C) p-Chk2 expression was assessed in ATM-specific inhibitor KU-55933-treated cells and (D) quantified by western blot analysis normalized to GAPDH. (E) SA-β-gal staining and (F) the percentage of SA-β-gal-positive cells. Data are expressed as the means ± SD of three independent experiments. *P<0.05 compared to the controls or negative group. KU, KU-55933.