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GATA4 is restored in CD40L-M-induced senescent cells. (A) Methylation-specific PCR for the GATA4 gene in A549, A549/TR and 16HBE cells. U represents amplification of unmethylated alleles; M represents methylated alleles. (B) GATA4 protein was measured using western blot analysis. (C) GATA4 expression was evaluated immunocytochemically. Data are expressed as the means ± SD of three independent experiments. *P<0.05, **P<0.01 compared to the controls or negative group.
