Figure 2.

YWHAZ downregulation increased cell proliferation and colony forming ability. (A and B) Transfection of Leni-YWHAZ (KD group) successfully downregulated YWHAZ expression in the 5637 and T24 bladder cancer cell lines. (C and D) Fluorescence showed that cells were successfully transfected with the lentivirus. (E) MTT assay showed that cell viability with OD490 value was significantly elevated in the Leni-YWHAZ (KD) groups after 3 days. Clone formation assay showed that a number of colonies were increased in the KD groups compared with the control groups after 14 days in the 5637 and T24 cells. (F) Quantitation of the colony numbers and (G) representative images of the colony formation assay in the KD and NC groups. KD, knockdown; NC, negative control. *P<0.05, **P<0.01.