Fig. 1.

Underdevelopment in pups from NSD3 transgenic females. a WT FVB/N and NSD3 transgenic females were mated with WT FVB/N males. All females produced normal size litters of 8–10 pups. At 9 days of age, pups nursed by NSD3 transgenic females (right) were already lagging behind developmentally compared to pups nursed by WT FVB/N females (left). b Side by side comparison photos show the underdevelopment of pups nursed by NSD3 transgenic females at the time of weaning or at 21 days of age. As seen on the left in all photos, these pups remained developmentally behind pups nursed by WT FVB/N females. c The bar graph shows the average weights of two litters of pups nursed by NSD3 transgenic females at days 9 and 21 compared to one litter of WT-nursed pups. NSD3-nursed pups were approximately half the weight of healthy pups at both time points compared to WT-nursed pups