Fig. 2.

Lactation deficiency in NSD3 transgenic female mice. a After euthanization, mammary glands were harvested from female mice at various time points. Virgin mammary glands were harvested from WT and NSD3 transgenic females at 12 weeks of age. NSD3 mammary glands showed increased ductal branching and terminal bud formation. b Mammary glands were harvested from females at mid-pregnancy between day 12 and 14 post-mating. Mammary glands from pregnant NSD3 mice showed more numerous and densely packed alveolar buds compared to mammary glands harvested from pregnant WT females. c Lactating mammary glands were harvested from WT and NSD3 transgenic females 7–10 days following the birth of litters. NSD3 mammary glands showed failure of the glands to functionally differentiate compared to WT mammary glands. d Two weeks were allowed post-weaning for involution to occur before harvesting mammary glands from WT and NSD3 females. Staining shows that mammary glands from NSD3 transgenic females failed to involute, while the WT mammary glands had undergone involution