Figure 3.
Analysis of peroxidase purification fractions. A, Activity staining of purified peroxidases P7.3, P8.8, and P9.3 after IEF. pH values calculated using pI markers are shown on the left. B, Immunoblot analysis. Protein was detected using polyclonal anti-rPrx7 antibodies. Analysis of rPrx7. Lane 1, Protein not bound to cation-exchange chromatography (ResS) or anion-exchange chromatography (ResQ), captured on phenyl ether-HIC, and containing P7.3; lane 2, pooled 30% elution buffer eluate from ResS; lane 3, pooled 15% elution buffer eluate from ResS; and lane 4, activity bound on and eluted from ResQ. C, SDS-PAGE of P9.3 and P7.3. The gel was stained with Coomassie Blue. Molecular mass markers (×103) are shown on the right.