Figure 1. Schematic illustration of study design.

BWH, Brigham and Women’s Hospital; CTLA-4, cytotoxic t-lymphocyte associated protein 4; CyTOF, time-of-flight mass cytometry; MPM, malignant pleural mesothelioma; MSKCC, Memorial Sloan Kettering Cancer Center; PD-1, programmed cell death 1; SCAFFOLD, single-cell analysis by fixed force– and landmark-directed; SPADE, spanning-tree progression analysis of density-normalized events; TCGA, The Cancer Genome Atlas; and TiME, tumor immune microenvironment.