Figure 3. AAV–IL-27 treatment enhances T cell effector functions and depletes Tregs.

B16.F10 cells (2 × 10⁵) (A–E) or 1 × 10⁶ MC38 cells (F–H) were injected into C57BL/6 mice s.c. Four days later, mice were treated with AAV–IL-27 or AAV-ctrl virus. Mice were sacrificed on day 21, and their tumors were quantified for percentage of CD4⁺ and CD8⁺ T cells among total tumor mononuclear cells (A and F), expression of IFN-γ (B and G), Granzyme B (C), and Perforin (D) in tumor-infiltrating T lymphocytes. Proportions of FoxP3⁺ Tregs (E and H) in spleens and tumors from AAV-treated mice were also quantified. Data are expressed as mean ± SEM and represent 2–5 experiments with similar results. *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t test.