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. 2018 Mar 8;8(8):2161–2170. doi: 10.7150/thno.23335

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Tissue processing and tissue section analysis (patient #8). Thin (5-10 mm) tissue slices (A) of the resected specimens were analyzed by autoradiography (B) and near-infrared fluorescence imaging (C). Both the radioactive and fluorescence signals were localized in tumor tissue. Histopathology of a tissue section showed homogeneous expression of CAIX in tumors coinciding with the distribution of the fluorescence signal. (D) H&E staining with the tumor annotated. (E) M75-staining showed a high and homogenous expression of CAIX in the tumor, visually overlapping with the distribution of the fluorescence signal. (F) Fluorescence imaging of a tissue section showed that T:N contrast is high at the tumor borders.

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