Table 3.
Serum biochemical parameters of LF and HF diet-fed C57BL/6J mice
| Parameter | LF (2w) | HF (2w) | LF (4w) | HF (4w) | LF (8w) | HF (8w) | LF (12w) | HF (12w) |
|---|---|---|---|---|---|---|---|---|
| CHOL (mM) | 3.57±0.33 | 4.22±0.23** | 4.08±0.16 | 5.91±0.17** | 3.96±0.26 | 6.16±0.19** | 3.83±0.29 | 7.17±0.35** |
| FBG (mM) | 4.52±0.32 | 4.58±0.43 | 4.33±0.33 | 4.92±0.29 | 4.65±0.54 | 5.42±0.39 | 4.81±0.16 | 5.69±0.28* |
| HOMA-IR | 1.39±0.11 | 1.51±0.16 | 1.48±0.11 | 2.28±0.20** | 2.24±0.33 | 4.43±0.44** | 2.42±0.07 | 6.91±0.29** |
| INS (mIU/L) | 6.93±0.22 | 7.44±0.82 | 7.74±0.14 | 10.46±0.80** | 10.62±0.58 | 18.31±1.11** | 11.34±0.25 | 27.52±0.52** |
| KITT (%/min) | 3.27±0.27 | 3.23±0.36 | 3.14±0.31 | 3.09±0.25 | 3.34±0.35 | 3.17±0.10 | 3.13±0.25 | 1.79±0.43** |
| TAG (mM) | 0.95±0.09 | 1.03±0.10 | 1.13±0.07 | 1.35±0.09* | 1.14±0.07 | 1.42±0.06* | 1.28±0.07 | 1.87±0.07** |
Mice were treated with LF or HF diet for 2, 4, 8 and 12 weeks, and FBG, TAG, CHOL, INS, HOMA-IR and KITT were determined. HF: high-fat diet group; LF: low-fat diet group. The interactions between week and group were cholesterol (CHOL; F=5.485, p<0.05), fasting blood glucose (FBG; F=0.088, p>0.05), homeostasis model assessment of insulin resistance (HOMA-IR; F=28.025, p<0.01), insulin (INS; F=46.773, p<0.01), the slope of insulin tolerance test (KITT; 0-15 min; F=3.157, p<0.05) and triglyceride (TAG; F=4.579, p<0.05). Analyses were performed by two-way ANOVA, followed by a post-hoc Bonferroni test to determine individual differences among groups. Values are presented as mean ± SEM, n=10, *p<0.05 and **p<0.01 compared to the corresponding LF group.