Figure 2.

Treatment of Kasumi-1 cells with Bet (50 nM) affected R-E/RUNX1-mediated gene expression and induced differentiation and apoptosis. (A-C) GSEA plots show the drug treatment caused significant (A) up- and (B) down-regulation of the R-E up- and down-regulated gene sets, as well as significant (C) upregulation of the RUNX1-upregulated gene set. (D) qPCR results show Bet caused significant expression changes of selected R-E/RUNX1 target genes. (D) The drug treatment caused significantly increased cell populations expressing high levels of CD11b and CD14. (E) Treatment with 30 nM and 100 nM of Bet caused 6.9% and 21.8% apoptosis (as compared to the control).