Figure 4.

Western blot and immunoprecipitation results. (A) Treatment of Kasumi-1 cells with Bet caused significant changes of GR, RUNX1 and R-E in the cytoplasm and nucleus. (B) Immunoprecipitation showed GR is associated with RUNX1, but not R-E in Kasumi-1. (C) Upon transfection of Flag-RUNX1 or Flag-R-E, immunoprecipitation using a Flag antibody also show that GR is associated with RUNX1, but not R-E in 293T cells. (D) Illustrations showing different constructs of RUNX1 used for transfection. (E) Immunoprecipitation results show that WT-, Δ429- and Δ391-RUNX1 is associated with GR, while Δ321-RUNX1 does not interact with GR.