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. 2018 Mar 8;8(8):2202–2216. doi: 10.7150/thno.24003

Figure 4.

Figure 4

OVOL2 negatively regulates the tumorigenicity of NPC. (A) OVOL2 knockout (KO) in CNE2 cells increased the ability to form colonies on conventional plates. Mean ± SD, n = 3. (B) Colony formation of CNE2 WT, OVOL2-KO and OVOL2-KO cells reconstituted with ectopic OVOL2 (n = 3). (C) EMT marker expression in CNE2 cells stably expressing OVOL2 or empty vector. (D) Serial transplantation experiments using limiting dilutions of WT or OVOL2-KO CNE2 cells (n = 8 mice for each dilution). (E) CNE2 cells stably expressing OVOL2 or empty vector were subcutaneously injected into nude mice, and images were taken 22 days post-implantation. (F) Tumor weight in mice injected s.c. with CNE2 control or OVOL2-overexpressing cells (1×105 cells; n = 8). (G) Growth curves of xenograft tumors formed by CNE2 cells stably expressing OVOL2 or empty vector (1×105 cells were inoculated s.c.; n = 8). (H) WT or OVOL2-KO CNE2 cells were subcutaneously injected into nude mice, and images were taken 22 days post-implantation. (I) Tumor weight in mice injected with CNE2 WT or OVOL2-KO cells. (J) Growth curves of xenograft tumors formed by WT or OVOL2-KO CNE2 cells (5×104 cells were inoculated s.c.; n = 6).