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. 2018 Mar 22;8(9):2361–2376. doi: 10.7150/thno.23438

Figure 4.

Figure 4

RFP electroporation in the skin. (A) The whole-body fluorescence intensity imaging indicates efficient RFP plasmid expression in electroporated mouse skin. Animals received the following treatments: (G1) without any treatment as negative control; (G2) electroporation with plate electrode at 100 V; (G3) electroporation with FIEA at 50 V without rolling; (G4) electroporation with FIEA at 50 V with rolling; (G5) electroporation with FIEA at 70 V without rolling; (G6) electroporation with FIEA at 70 V with rolling. Fluorescence images were merged with X-ray images to show the fluorescent sites. Electroporation conditions were: 10 electrical pulse; voltage 100, 50 or 70 V; 10 ms pulse duration; 1 s pulse interval. (B) Enlarged images from (A) indicating the electroporation areas of the mice. (C) Grouping information. (D) Quantitative analysis of (A) or (B). The mean fluorescence intensity (MFI) was calculated from 4 different mice. Data were normalized to an unelectroporated mouse who's MFI was set to zero. Each bar represents the mean ± SEM. Statistical analyses were performed using two-tailed Student's t-test. *, vs. unelectroporated mice at the same time point, *P <0.05, **P <0.01, and ***P <0.001. #, vs. mice electroporated with plate electrodes at the same time point, #P <0.05, ##P <0.01, and ###P <0.001. &, vs. mice electroporated at the same voltage with FIEA but without rolling at the same time point, &P <0.05, &&P <0.01, and &&&P <0.001.