Figure 1.

(A, B) Fluorescence kinetic curves of mutation base in the middle, distal or proximal site of the target and target detection by ExP and OrP, respectively. (C) Relative fluorescence intensity of the ExP and OrP in detecting single-base mutation target and wild-type target. (D) Corresponding discrimination factors (DFs) of ExP and OrP. (E) UV-vis characterization of the stability of Au-TDNNs in SSC, PBS and DMEM (10% FBS). (F) Zeta potential analysis of the step-by-step assembly of the Au-TDNNs. RFI: relative fluorescence intensity. DF = ∆F_target/∆F_{target analogue} (∆F = F-F₀, F₀: background signal.). *P < 0.001.