Figure 3.

(A) Feasibility of Au-TDNNs was validated by measuring the fluorescence enhancement on hybridizing with target, miR-200b and combination of different components. (B) Relative fluorescence intensity of Au-TDNNs after hybridization with different concentrations of target. Target concentrations were from 1 nM to 100 nM and the fluorescence was collected at 520 nm. (C) The ability of Au-NPs@ExP1P2 and Au-NPs@OrP1P2 to distinguish point mutation target and target analogs were evaluated by DF. (D) MiR-21 detection in MCF-7 cell-derived total RNA samples. *P < 0.001.