Figure 2.

Harmine augments the pro-angiogenic effects of preosteoclasts on endothelial cells. (A-D) Representative images and quantification of tube formation in HMECs stimulated with CM from different groups and PDGF-BB-neutralizing antibody or IgG isotype control antibody. Scale bar: 200 μm. n = 5 per group. (E) CCK-8 analysis of HMEC proliferation in different treatment groups. n = 4 per group. (F-I) HMEC motility in different treatment groups was evaluated by the transwell migration assay (F-G) (Scale bar: 100 μm) and the scratch wound assay (H-I) (Scale bar: 200 μm). n = 3 per group. *P < 0.05 vs. CM^Vehicle + IgG group, ^#P < 0.05 vs. CM^RANKL + IgG group, ^△P < 0.05 vs. CM^{RANKL + Harmine} + IgG group.