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. 2018 Mar 28;8(9):2477–2487. doi: 10.7150/thno.24173

Figure 4.

Figure 4

Verification of roles of autophagy and importin α/β on nuclear import and cell death. (A) Immunofluorescence microscopy for LC3/importin α and LC3/importin β co-localization. (B) Necrosis and apoptosis. The data represent mean ± s.d of at least 3 independent culture experiments. Skov-3 cells were treated with scramble siRNA as the control or siRNAs to knockdown ULK1, ATG7, SNAP-29, KPNA (encoding importin α) or KPNB (encoding importin β). The siRNA-transfected cells were treated with GO/CDDP for 24 h and subjected to immunofluorescence microscopy. The cells were also detached for Annexin V/7-AAD labeling and flow cytometry analysis to measure necrosis and apoptosis. The data represent mean ± s.d of at least 3 independent culture experiments.