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. 2018 Apr 3;8(9):2565–2582. doi: 10.7150/thno.22878

Figure 2.

Figure 2

Overexpression of miR-378 in cardiac fibroblasts inhibits collagen deposition and cardiac fibroblasts proliferation. (A) Cultured rat cardiac fibroblasts were transiently transfected with the chemically modified miR-378 mimics (35 nM) or negative scramble control (mock). The effect of miR-378 on fibroblast proliferation was measured by MTT assay. (B) Quantitative real-time polymerase chain reaction analysis of collagen Iα1 and collagen IIIα1 mRNA expression in CFs. (C) Western blot analysis of MMP9 in CFs. Cells were transfected by miR-378 mimics or mock scrambles and subjected to mechanical stretching (MS) for 24 h. (D) Representative photos of CFs analyzed by EdU incorporation assay (scale bar: 50 μm). Red color indicates the EdU-positive cells; blue color indicates DAPI-positive cells. The EdU incorporation rate is shown as the radio of EdU-positive cells to total DAPI-positive cells. Data are from three independent experiments. DAPI indicates 4',6-diamidino-2-phenylindole. Values represent the mean ± SE from three independent experiments. **, P< 0.01 vs. mock; *, P< 0.05 vs. mock; ##, P< 0.01 vs. mock subjected to MS.