Figure 3.

Sophora flavescens alkaloid gels induces the apoptosis and cell cycle arrest in cervical cancer cells. (A) Flow cytometry apoptosis analysis was performed in cultured Siha, C33A and Ca ski cell lines harvested after 2 days of treatment with different concentration of SFA gels, matrine and oxymatrine. (B) Quantification of apoptosis for the cell lines. *P<0.05, **P<0.01. Columns are means of three independent experiments, bars are SD. (C) Cell cycle analysis was performed using flow cytometry by staining with propidium iodide. (D) Columns represent quantification of cell phase. (E) and (F) Western Blot analysis of apoptosis-associated and cell cycle regulatory proteins.