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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Cell Rep. 2018 Apr 17;23(3):701–708. doi: 10.1016/j.celrep.2018.03.094

Figure 2. TMEM150C Potentiates MA Current Produced by Piezo1.

Figure 2

(A) Exemplar whole-cell MA current traces recorded in HEK293TΔP1 cells in response to mechanical indentation with a glass probe at Ehold = −80 mV. Arrow indicates the position of persistent post-stimulus MA current measurement.

(B) Peak MA current measured at different indentation depths in HEK293TΔP1 cells expressing indicated constructs (Ehold = −80 mV). Data shown as mean ± SEM.

(C and D) Quantification of MA current activation threshold (C) and inactivation rate τinact (D). NS, not significant; p > 0.05, **p < 0.01; unpaired t test (C) and Mann-Whitney U-test (D). Data shown as mean ± SEM.

(E) Post-stimulus MA current amplitude at different indentation depths. ****p < 0.0001 for expression construct effect, two-way ANOVA. Data shown as mean ± SEM.

(F) Quantification of peak-normalized amplitude of persistent post-stimulus MA current. ****p < 0.0001, Mann-Whitney U test. Data shown as mean ± SEM.

See also Figure S2A.