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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Cell Rep. 2018 Apr 17;23(3):701–708. doi: 10.1016/j.celrep.2018.03.094

Figure 3. TMEM150C Potentiates Piezo1 MA Current Evoked by High-Speed Pressure Clamp.

Figure 3

(A) Exemplar cell-attached MA current traces recorded in HEK293TΔP1 cells in response to application of a negative pressure in the pipette at Ehold = −60 mV. Each pressure step was preceded by a 500-ms step at 5 mmHg to remove inactivation. Arrow indicates the position of persistent post-stimulus MA current measurement.

(B) Quantification of peak MA current amplitude measured at −60 mV at different pressures. Data shown as mean ± SEM.

(C and D) Quantification of MA current activation threshold (C) and inactivation rate τinact (D). NS, not significant; p > 0.05, **p < 0.01; Mann-Whitney U-test (C) and unpaired t test (D). Data shown as mean ± SEM.

(E) Post-stimulus MA current amplitude at different pressures. ****p < 0.0001 for expression construct effect, two-way ANOVA. Data shown as mean ± SEM.

(F) Quantification of peak-normalized amplitude of persistent post-stimulus MA current. *p < 0.05, Welch’s test. Data shown as mean ± SEM.

See also Figure S2B.