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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cell Stem Cell. 2018 Mar 1;22(3):428–444.e5. doi: 10.1016/j.stem.2018.02.005

Figure 2. SETD7 is necessary for CM differentiation.

Figure 2

(A) Immunoblot analysis of cell lysates from HES3NKX2-5eGFP/w hESC clone transfected with TALEN targeting start codon of SETD7 gene (upper). Immunoblot analysis of cell lysate from SETD7+/+ and SETD7−/− HES3NKX2-5eGFP/w-derived CMs (lower) (n=3). (B) NKX2-5-GFP expression in SETD7+/+ and SETD7−/− HES3NKX2-5eGFP/w-derived CMs (left). Flow cytometry of GFP+ cells (right). Scale bar, 100 µm. (C) RT-qPCR of TBX20 and MYH6 in SETD7+/+ and SETD7−/− HES3NKX2-5eGFP/w-derived CMs. (D) mRNA levels of SETD7, TNNT2, and MYH6 in control and SETD7 shRNA hESC-CMs. hESCs were infected with lentivirus carrying scramble or SETD7 shRNA. (E–F) Expression level of cardiac-related genes in hESCs and hESC-CMs expressing doxycycline-inducible scramble or SETD7 shRNA. (G) Representative fluorescence microscopy images of hESC HES3NKX2-5eGFP/w-derived CMs. Scale bar, 100 µm. Statistical significance obtained by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.005 (mean ± SEM; n=4). See also Figure S2.