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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Cell Stem Cell. 2018 Mar 1;22(3):428–444.e5. doi: 10.1016/j.stem.2018.02.005

Figure 7. SETD7 is critical for calcium handling properties of mature cardiomyocytes.

Figure 7

(A) SETD7 expression at various days of hESC-CM differentiation (n=4). (B–C) RT-qPCR of SETD7 and TNNT2 expression levels in hESC, hESC-CMs, fetal heart, and adult heart tissues. (D) Immunoblot analysis of SETD7 in control and SETD7 knock-down lines. (E) Cell viability assay of control and SETD7 knock-down lines. (F) Standard deviation of peak intervals of control and SETD7 knock-down lines. Motion vector from movie images was recorded and analyzed with SONY SI8000 cell motion imaging system (n=18). (G) Total number of wells with arrhythmic-beating hESC-CMs from control and SETD7 knock-down lines. (H) Representative traces of the motion velocity of hESC-CMs infected with scramble or SETD7 shRNA. (I) Immuno-staining of Cxn43, α-Actinin and TNNT2 in control and SETD7 knock-down CMs. (J) Representative ratio-metric calcium imaging traces of control and SETD7 knock-down hESC-CMs. (K–Q) Calcium handling parameters of scramble and SETD7 shRNA lines (n>30). (R–T) Relative RNA expression of SETD7 and calcium handling genes in scramble and shRNA SETD7 groups. (U) ChIP-qPCR of SETD7 and IgG enrichment on TNNT2, MYH6, and CASQ2 genes in hESC-CMs (n=3). Statistical significance obtained by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.005 (mean ± SEM).