Figure 3. Pharmacological inhibition and silencing of AR in glioma cell lines.

(A-C) GBM cell lines were maintained in culture medium supplemented with androgens (as well as other steroids)-stripped FBS. (D, E) GIC cell lines were treated with neurobasal medium without FBS. (A-E) both GBM cell lines and GIC cell lines were treated with vehicle (0.15% ethanol) (white bars with black dots) or with a physiological dose (10nm) of DHT alone (gray bars) or in combination with the indicated doses of bicalutamide (BIC, black bars) or enzalutamide (ENZ, white bars) (X-axis) for 72 hrs. Cell viability was determined as described in Methods and is expressed as the percentage of viable cells following treatment with DHT (Y-axis). (A) A172 cell line; (B) U87MG cell line; (C) T98G cell line; (D) ZH-161cell line; (E) ZH-305 cell line. (F-G) Cells were transfected with 1.2 nM of non-targeted siRNA (control siRNA) or siRNAs targeting human AR. (F) RNA interference determined by Quantitative real-time RT PCR 24 hrs later. (G) Cell viability determined with the crystal violet assay 72h later. All experiments were repeated at least three times. The results of the viability experiments are presented as the mean ± SD ^*P<0.05, ^**P<0.01 versus control group.