Figure 2. NTZ inhibits the VACV replicative cycle after entry.

HF were infected with VACV at an MOI of 3 and mock treated or treated with 10 μM NTZ at 1h pi. (A) E3L transcript abundance, relative the amount present at 1 hpi, was quantified by qRT-PCR as described in the Methods. (B) Proteins were labelled by incubating cells with 100 μCi/ml of S³⁵-methionine for 1h before collecting cell lysates and were analysed by SDS-PAGE and autoradiography. (C) Cell lysates were collected at the indicate times timepoints and the accumulation of late VACV proteins D8 and A14 was monitored via immunoblot assay. (D) The fold increase in VACV DNA at 24 hpi, compared to amount present at 1 hpi, was measured by qPCR. (* p < 0.05; ** p < 0.005; ns, not significant)