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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Apr 3;518:398–405. doi: 10.1016/j.virol.2018.03.023

Figure 4. NTZ does not act by cGAS/STING or IRF3 signaling.

Figure 4

(A) HF or HF STING−/− were infected with VACV (MOI = 0.1) and mock treated or treated with 10 μM NTZ at 1 hpi. Viral production was determined at 48 hpi by β-gal expression (ns, not significant). (B) HF were mock infected or infected with VACV (MOI = 3) and mock treated or treated with 10 μM NTZ at 1 hpi. UV-inactivated HCMV was used as a control for IRF3 translocation to the nucleus. Nuclear-cytosolic fractionation was performed at 4 hpi, and IRF3 distribution was determined by immunoblot assays. H3 was used as a control for the nuclear fraction, and STING expression for the cytosolic fraction.