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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Apr 3;518:398–405. doi: 10.1016/j.virol.2018.03.023

Figure 5. α-KG supplementation does not rescue VACV production after treatment with NTZ.

Figure 5

(A) HF were infected with VACV (MOI = 0.1) in DMEM either containing or lacking glutamine (Gln), and treated at 1hpi with either 10 μM NTZ or 7mM α-KG, or a combination of both in the respective media. Viral production was determined at 48 hpi by β-gal expression. (B) HF were infected with VACV (MOI = 0.1) and treated with 10 μM NTZ, 20 μM Z-VAD-FMK (zVAD), 10 μM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), 1 μM antimycin A (AM), 2.5 μM oligomycin (OM), 400 μM 2,4-dinitrophenol (DNP), or a combination of 10 μM NTZ and 20 μM zVAD. Viral production was measured at 48 hpi by β-gal expression.